Ten patients in the study group have developed anti-SARS-CoV-2 antibodies. One patient presented with mild COVID-19 symptoms.

While our study had a small sample size, ongoing biologic treatment in psoriasis was not associated with severe form of COVID-19.

While our study had a small sample size, ongoing biologic treatment in psoriasis was not associated with severe form of COVID-19.The human pathogen Bordetella pertussis targets the respiratory epithelium and causes whooping cough. Selleckchem PD166866 Its virulence factor adenylate cyclase toxin (CyaA) plays an important role in the course of infection. Previous studies on the impact of CyaA on human epithelial cells have been carried out using cell lines derived from the airways or the intestinal tract. Here, we investigated the interaction of CyaA and its enzymatically inactive but fully pore-forming toxoid CyaA-AC- with primary human airway epithelial cells (hAEC) derived from different anatomical sites (nose and tracheo-bronchial region) in two-dimensional culture conditions. To assess possible differences between the response of primary hAEC and respiratory cell lines directly, we included HBEC3-KT in our studies. In comparative analyses, we studied the impact of both the toxin and the toxoid on cell viability, intracellular cAMP concentration and IL-6 secretion. We found that the selected hAEC, which lack CD11b, were differentially susceptible to both CyaA and CyaA-AC-. HBEC3-KT appeared not to be suitable for subsequent analyses. Since the nasal epithelium first gets in contact with airborne pathogens, we further studied the effect of CyaA and its toxoid on the innate immunity of three-dimensional tissue models of the human nasal mucosa. The present study reveals first insights in toxin-cell interaction using primary hAEC.The mechanisms by which Y chromosome microdeletions cause infertility have been well described; however, the therapeutic targets remain a challenge. Here, we used whole-genome sequencing to explore the mechanism of Y chromosome deletion and potential therapeutic targets in a patient with infertility. There were no abnormalities in the patient’s medical history. Routine semen analysis showed immotile sperm and only two motile spermatozoa were occasionally see after centrifugation, indicating that the direct cause of infertility was an abnormal sperm count and motility. A Y chromosome microdeletion test revealed partial deletion of the AZFc region, including AZFc1, AZFc2, AZFc3, and AZFc4. Whole-genome sequencing showed that the patient had seven harmful mutations, with only one significant epigenetic mutation, SH3KBP1. Gene Ontology analysis of these meaningful mutations indicated involvement of cAMP signaling pathways. The patient’s wife became pregnant following in vitro fertilization, and no significant abnormalities were found during prenatal examination. This case suggests that Y chromosome microdeletion and gene mutation may affect the cAMP signaling pathway, leading to reduced sperm quality and male infertility.Fructus Psoraleae (FP), widely used in traditional medicine, is increasingly reported to cause serious hepatotoxicity in recent years. However, the main toxic constituents responsible for hepatotoxicity and the underlying mechanisms are poorly understood. In the present study, psoralen, a main and quality-control constituent of FP, was intragastrically administered to Sprague-Dawley rats at a dose of 60 mg/kg for 1, 3 and 7 days. Blood and selected tissue samples were collected and analyzed for biochemistry and histopathology to evaluate hepatotoxicity. The results showed that psoralen could induce hepatotoxicity by enhanced liver-to-body weight ratio and alterations of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total cholesterol after administration for 3 days. In addition, histopathological examinations also indicated the hepatotoxicity induced by psoralen. Furthermore, the mRNA and protein levels of hepatic bile acid transporters were significantly changed, in which MRP4, ABCG5 and ABCG8 were repressed, while the protein level of NTCP tended to increase in the rat liver. Taken together, psoralen caused liver injury possibly through affecting bile acid transporters, leading to the disorder of bile acid transport and accumulation in hepatocytes.The aim of this study was twofold. Firstly, it explored the relationships between morningness, Big Five personality traits, depression, and problematic Internet use (PIU). Secondly, it examined the possible mediating role of depression in these relationships. The following measures were used the Internet Addiction Test, the Composite Scale of Morningness, the International Personality Item Pool-Big Five Markers-20, and the Center for Epidemiologic Studies Depression Scale. The participants in the study were 398 university students 18 to 30 (M = 20.37, SD = 2.29) years of age. The study found that PIU Total, PIU Mental Disorder, and PIU Time Management Disorder are positively related to depression and negatively related to morningness, Extraversion, Agreeableness, Conscientiousness, Emotional Stability, and Openness. PIU was more often found in individuals with an evening chronotype. The study confirmed the role of depression as a mediator in the relationship of personality traits and morningness to PIU Mental Disorder and PIU Time Management Disorder. For PIU Mental Disorder, the study revealed a full mediation effect of depression in the case of morningness, Emotional Stability, and Extraversion, and partial mediation in the case of Agreeableness and Intellect. For PIU Time Management Disorder, there was a full mediation effect of depression in the case of Emotional Stability, Extraversion, and Agreeableness, and partial mediation in the case of morningness and Intellect. The results highlight the importance of including depression when investigating the relationship between morningness and personality in studies of PIU.β-Thalassemia (β-thal) is one of the most common inherited diseases all over the world. These patients are very susceptible to infection disease, and the mortality and morbidity of infected patients will increase. The object of this systematic review study was to determine mortality and morbidity of infected β-thal patients with coronavirus disease 2019 (COVID-19). We searched PubMed, Elsevier, and Scholar Google to obtain related papers. The time of search was 21 June until 17 July 2020. All original and review articles and case reports were searched with key words COVID 19, beta or β-thalassemia (β-thal), mortality and morbidity. Data were extracted after quality assessment of all articles. We obtained seven, 21 and six articles from PubMed, Scholar Google and Science Direct, respectively. Finally, seven articles were discussed in our study. The total number of enrolled patients was 34. Twenty-six patients carried transfusion-dependent β-thal major (β-TM). The most common symptoms were fever, cough, pain and dyspnea.