Thus, Sias on glycoconjugates of the intact glycocalyx and its desialylation are major regulators of neuroinflammation.Hepatitis B virus (HBV) polymerase seems to be very hard to express and purify sufficiently, which has long hampered the generation of anti-HBV drugs based on the nature of the polymerase. To date, there has been no useful system developed for drug screening against HBV polymerase. In this study, we successfully obtained a highly purified reverse transcriptase (RT) domain of the polymerase, which has a template/primer and substrate binding activity, and established a novel high-throughput screening (HTS) system using purified RT protein for finding novel polymerase inhibitors. To examine whether the assay system provides reliable results, we tested the small scale screening using pharmacologically active compounds. As a result, the pilot screening identified already-known anti-viral polymerase agents. Then, we screened 20,000 chemical compounds and newly identified four hits. Several of these compounds inhibited not only the HBV RT substrate and/ template/primer binding activity, but also Moloney murine leukemia virus RT activity, which has an elongation activity. Finally, these candidates did show to be effective even in the cell-based assay. PEG400 solubility dmso Our screening system provides a useful tool for searching candidate inhibitors against HBV.Artemisinin combination therapy (ACT) is recommended by the World Health Organization (WHO) as first line treatment for uncomplicated malaria both in adults and children. During pregnancy, ACT is considered safe only in the second and third trimester, since animal studies have demonstrated that artemisinin derivatives can cause foetal death and congenital malformation within a narrow time window in early embryogenesis. During this period, artemisinin derivatives induce defective embryonic erythropoiesis and vasculogenesis/angiogenesis in experimental models. However, clinical data on the safety profile of ACT in pregnant women have not shown an increased risk of miscarriage, stillbirth, or congenital malformation, nor low birth weight, associated with exposure to artemisinins in the first trimester. Although further studies are needed, the evidence collected up to now is prompting the WHO towards a change in the guidelines for the treatment of uncomplicated malaria, allowing the use of ACT also in the first trimester of pregnancy.With smart city infrastructures growing, the Internet of Things (IoT) has been widely used in the intelligent transportation systems (ITS). The traditional adaptive traffic signal control method based on reinforcement learning (RL) has expanded from one intersection to multiple intersections. In this paper, we propose a multi-agent auto communication (MAAC) algorithm, which is an innovative adaptive global traffic light control method based on multi-agent reinforcement learning (MARL) and an auto communication protocol in edge computing architecture. The MAAC algorithm combines multi-agent auto communication protocol with MARL, allowing an agent to communicate the learned strategies with others for achieving global optimization in traffic signal control. In addition, we present a practicable edge computing architecture for industrial deployment on IoT, considering the limitations of the capabilities of network transmission bandwidth. We demonstrate that our algorithm outperforms other methods over 17% in experiments in a real traffic simulation environment.This work establishes a tensegrity model of spider dragline silk. Tensegrity systems are ubiquitous in nature, being able to capture the mechanics of biological shapes through simple and effective modes of deformation via extension and contraction. Guided by quantitative microstructural characterization via air plasma etching and low voltage scanning electron microscopy, we report that this model is able to capture experimentally observed phenomena such as the Poisson effect, tensile stress-strain response, and fibre toughness. This is achieved by accounting for spider silks’ hierarchical organization into microfibrils with radially variable properties. Each fibril is described as a chain of polypeptide tensegrity units formed by crystalline granules operating under compression, which are connected to each other by amorphous links acting under tension. Our results demonstrate, for the first time, that a radial variability in the ductility of tensegrity chains is responsible for high fibre toughness, a defining and desirable feature of spider silk. Based on this model, a discussion about the use of graded tensegrity structures for the optimal design of next-generation biomimetic fibres is presented.To solve the problem of vibration motor fault detection accuracy and inefficiency in smartphone components, this paper proposes a fault diagnosis method based on the wavelet packet and improves long and short-term memory network. First, the voltage signal of the vibration motor is decomposed by a wavelet packet to reconstruct the signal. Secondly, the reconstructed signal is input into the improved three-layer LSTM network as a feature vector. The memory characteristics of the LSTM network are used to fully learn the time-series fault feature information in the unsteady state signal, and then, the model is used to diagnose the motor fault. Finally, the feasibility of the proposed method is verified through experiments and can be applied to engineering practice. Compared with the existing motor fault diagnosis method, the improved WP-LSTM diagnosis method has a better diagnosis effect and improves fault diagnosis.Previous studies have reported filarial parasites of the genus Dipetalonema and Mansonella from French Guiana monkeys, based on morphological taxonomy. In this study, we screened blood samples from nine howler monkeys (Alouatta macconnelli) for the presence of filaria and Wolbachia DNA. The infection rates were 88.9% for filaria and 55.6% for wolbachiae. The molecular characterization, based on the 18S gene of filariids, revealed that A. macconnelli are infected with at least three species (Mansonella sp., Brugia sp. and an unidentified Onchocercidae species.). Since the 18S and cox1 generic primers are not very effective at resolving co-infections, we developed ITS genus-specific PCRs for Mansonella and Brugia genus. The results revealed coinfections in 75% of positives. The presence of Mansonella sp. and Brugia sp. was also confirmed by the 16S phylogenetic analysis of their associated Wolbachia. Mansonella sp., which close to the species from the subgenus Tetrapetalonema encountered in New World Monkeys, while Brugia sp.