Collectively, these results indicate that Dek605 specifically affects the single nad1-608 site and is required for normal seed development and resulted in nutritional quality relevant amino acid accumulation.

Drug immunomodulation modifies the response of the immune system and can be therapeutically exploited in pathologies such as cancer and autoimmune diseases.

DREIMT is a new hypothesis-generation web tool which performs drug prioritization analysis for immunomodulation. DREIMT provides significant immunomodulatory drugs targeting up to 70 immune cells subtypes through a curated database that integrates 4,960 drug profiles and ∼2,6K immune gene expression signatures. The tool also suggests potential immunomodulatory drugs targeting user-supplied gene expression signatures. Final output includes drug-signature association scores, FDRs and downloadable plots and results tables.

http//www.dreimt.org.

Supplementary data are available at Bioinformatics online.

Supplementary data are available at Bioinformatics online.A fundamental aspect of mitochondria is that they possess DNA and protein translation machinery. Mitochondrial DNA encodes 22 tRNAs that translate mitochondrial mRNAs to 13 polypeptides of respiratory complexes. Various chemical modifications have been identified in mitochondrial tRNAs via complex enzymatic processes. A growing body of evidence has demonstrated that these modifications are essential for translation by regulating tRNA stability, structure and mRNA binding, and can be dynamically regulated by the metabolic environment. Importantly, the hypomodification of mitochondrial tRNA due to pathogenic mutations in mitochondrial tRNA genes or nuclear genes encoding modifying enzymes can result in life-threatening mitochondrial diseases in humans. Thus, the mitochondrial tRNA modification is a fundamental mechanism underlying the tight regulation of mitochondrial translation and is essential for life. In this review, we focus on recent findings on the physiological roles of 5-taurinomethyl modification (herein referred as taurine modification) in mitochondrial tRNAs. We summarize the findings in human patients and animal models with a deficiency of taurine modifications and provide pathogenic links to mitochondrial diseases. We anticipate that this review will help understand the complexity of mitochondrial biology and disease.

Analysis of biological data often involves the simultaneous testing of thousands of genes. This requires two key steps the ranking of genes and the selection of important genes based on a significance threshold. One such testing procedure, called the ‘optimal discovery procedure’ (ODP), leverages information across different tests to provide an optimal ranking of genes. This approach can lead to substantial improvements in statistical power compared to other methods. However, current applications of the ODP have only been established for simple study designs using microarray technology. Here we extend this work to the analysis of complex study designs and RNA sequencing studies.

We apply our extended framework to a static RNA sequencing study, a longitudinal and an independent sampling time-series study, and an independent sampling dose-response study. Our method shows improved performance compared to other testing procedures, finding more differentially expressed genes and increasing power for enrichment analysis. Thus the extended ODP enables a superior significance analysis of genomic studies.

The algorithm is implemented in our freely available R package called edge and can be downloaded at https//www.bioconductor.org/packages/release/bioc/html/edge.html.

Supplementary data and code are available at https//github.com/StoreyLab/odp_general_studies.

Supplementary data and code are available at https//github.com/StoreyLab/odp_general_studies.

Orbital compartment syndrome (OCS) is a rare but devastating complication of over-resuscitation in burn patients that may lead to permanent visual loss. The purpose of this study was to (1) present a series of burn patients with OCS, and (2) survey practice patterns of monitoring intra-ocular pressure (IOP) during burn resuscitation.

Cases of OCS at two American Burn Association (ABA)-verified burn centers were retrospectively reviewed. Patients were included if they (a) required lateral canthotomy/cantholysis for elevated IOPs, or (b) developed blindness during admission unrelated to any other ocular pathology. this website Data was collected on demographics, burn characteristics, fluid administration, ophthalmologic findings and complications. An eight-item electronic survey was distributed by email through the ABA to all physician members.

Twelve patients with OCS were identified, with a mean age of 47.8 ±12.4 years and TBSA of 63.7 ±18.6%. Mean fluid resuscitation at 24 hours was 4.9 ±1.6mL/kg/%TBSA, or 0.29 ±0.lication of over-resuscitation.Clostridium estertheticum and C. estertheticum -like spp. are obligate anaerobic psychrophiles causing “blown pack” spoilage of chilled vacuum-packed meat. The present study aimed at detecting and isolating these spoilage bacteria in fecal samples of cattle of different ages at the slaughterhouse level. One-hundred-and-two swab fecal samples were obtained and enriched anaerobically in pre-reduced peptone-yeast-glucose-starch medium (PYGS) for three weeks at 4°C then screened for C. estertheticum and C. estertheticum -like spp. using a 16S rRNA gene-based real-time PCR assay (RT-PCR). The RT-PCR positive samples were further enriched for 3 weeks in PYGS then subjected to an ethanol (50% v/v) and lysozyme (4 mg/ml) treatment. Isolation was carried out anaerobically on Columbia Agar with 5% defibrinated sheep blood at 4°C for three weeks. Isolated strains were identified morphologically and by their 16S rRNA gene. Thirty-nine percent (n=40/102) of the samples were RT-PCR positive. The frequency of positive samples was 45% (n=9/20), 43% (n=23/54) and 29% (n=8/28) in calves, young cattle and cows/bulls aged ≤ 160, 161-1000 and >1000 days, respectively. Six strains were isolated from six out of the 40 RT-PCR positive samples. Out of these, five were from the calves (n=1) and young cattle (n=4). The six isolates were identified as C. estertheticum (n=1), C. frigoriphilum (n=1) and C. estertheticum -like spp. (n=4). The present findings confirm that feces of cattle are an important source of psychrophilic Clostridium spp. The fecal carriage among livestock animals at slaughter is strongly correlated with the risk of carcass contamination. Therefore, the maintenance of slaughter hygiene is of central importance.