The aim of this study was to verify the immediate effect of a flexible resonance tube in water and of lip trill on oropharyngeal geometry and vocal acoustic parameters of singers without vocal symptoms.

Twenty-two adult singers participated in the study. They had an average age of 27 (±4.8) years. Participants were split into two groups a group composed of 12 singers who performed the flexible resonance tube (FRT) exercise and a group of 10 singers who performed the Lip Trill technique (LTT). Acoustic pharyngometry and acoustic analysis of the voice were used to assess oropharyngeal geometry before and after the exercises.

After performing the techniques, the vocal tract length was longer in the group that performed the FRT, compared to the one that performed the LTT. In the acoustic evaluation, there was an improvement in the glottal to noise excitation ratio and a decrease in noise in the group of singers who performed the LTT. In the analysis by sex men had a longer oral cavity compared to women and after application of the techniques greater volume of the vocal tract.

There was variation in the oropharyngeal geometry with the FRT, while the LTT had a positive effect on the vocal acoustic parameters related to glottal noise.

There was variation in the oropharyngeal geometry with the FRT, while the LTT had a positive effect on the vocal acoustic parameters related to glottal noise.

To provide data on the measurable vocal fold vibratory differences in children with and without vocal fold lesions using high-speed videoendoscopy.

Prospective study, 24 participants (8 healthy; 16 with lesions) between the ages of 5 and 10.

Rigid high-speed videoendoscopy at the rate of 8,000 frames per second was used to examine participants. Four objective vocal fold phase linearity measures were obtained to establish anterior-posterior contact and separation vibratory patterns.

All objective measures showed a difference between nonlesion and bilateral vocal fold lesion groups. Contact-separation patterns in all nonlesion girls and young pre-pubertal boys exhibited an anterior-to-posterior contact and posterior-to-anterior separation; while older boys differed. The objective measures of open quotient, left-right relative phase asymmetry and speed index, showed linear anterior-posterior patterns within the nonlesion group; while the bilateral vocal fold lesion group displayed nonlinear patterns. Patrements of vocal fold vibration in presence of lesions. Further findings could help redefine the theoretical framework of pediatric voice.Moths often use multi-component pheromones with fixed ratios to keep intraspecific communication and interspecific isolation. Unusually, the Oriental armyworm Mythimna separata in North China use only Z11-16Ald as the essential component of its sex pheromone to find mates. To understand how this species keeps behavioral isolation from other species sharing Z11-16Ald as a major pheromone component, we study the olfactory coding of intra- and interspecific pheromonal messages in the males of M. separata. Firstly, we functionally characterized the long trichoid sensilla in male antennae by single sensillum recording. Two types of sensilla were classified the A type sensilla responded to Z11-16Ald and Z9-14Ald, and the B type sensilla mainly to Z9-14Ald, and also to Z11-16Ac, Z11-16OH, and Z9-16Ald. Next, we examined the glomerulus responses in the antennal lobes to these compounds by using in vivo optical imaging. The results showed that among the three subunits of the macroglomerular complex (MGC), Z11-16Ald acromone communication channel of the related species, our results suggest that the conserved olfactory pathways for behavioral antagonists play a crucial role in behavioral isolation of noctuid species.Present study deals with improvement in conventional technique of spraying liquid nitrogen on cancerous lesions to improve its efficacy. It is an attempt to demarcate the variation in outcome when cryogen is sprayed through customised multi-hole nozzle (MHN). Influence of mass flow rate on cryoablation while varying the number of holes in MHNs is analysed experimentally. Another section of study focuses on the impact of the rate of evaporation of cryogen when margin among the holes is changed. Total 6 MHNs are fabricated while changing their geometrical parameters. Single freeze-thaw cycle is carried out to spray liquid nitrogen on tissue mimicking gel with a spraying distance of 18 mm. Temperature profile captured through infrared images advocates that lethal area formed through the application of MHNs with 5 holes is larger than MHNs with 4 holes on the surface of gel. It reflects the influence of central hole on necrosis. In order to necrotize lesion through freezing, the most desired cooling rate (CR) varies from 50∘C/min to 200∘C/min. It is achieved up to a depth of 2 mm below the gel surface and in a radius of 10 mm from the centre of spray (CS) for all MHNs, except 4C. Most optimised margin in terms of cryoablation is also estimated in the study. this website MHN with 1.5 mm margin provides the most optimised results in terms of cryoablation when MHNs with the central hole are considered while MHN with 1 mm margin provides most optimised result when MHNs without central hole are considered. On the basis of observations made through thermal images it can be said that multiple freezing fronts are not observed on the gel surface which aids in the formation of lone giant ice ball. This provides an extra edge to the treatment methodology because alteration in the dimensions of necrotic zone can be made by changing the duration of spray. Larger lateral spread of necrotic zone will also reduce the number of sittings required for the treatment of larger lesions.In this study, the response surface methodology was used to optimize the cryoprotective agent (skimmed milk powder, lactose and sucrose) formulation for enhancing the viability of Lactobacillus curvatus N19 during freeze-drying and storage stability of cells freeze-dried by using optimum formulation was evaluated. Our results showed that the most significant cryoprotective agent influencing the viability of L. curvatus N19 to freezing and freeze-drying was sucrose and skim milk, respectively. The optimal formulation of cryoprotective agents was 20 g/100 mL skim milk, 3.57 g/100 mL lactose and 10 g/100 mL sucrose. Using the optimum formulation during freeze-drying, the cell survival was found more than 98%. Under the optimal conditions, although only storage of the cells at 4 °C for 6 month retained the maximum stability (8.85 log cfu/g), the employed protectant matrix showed promising results at 25 °C (7.89 log cfu/g). The storage stability of cells under optimized conditions was predicted by accelerated storage test, which was demonstrated that the inactivation rate constant of the freeze-dried L.