Moreover, co-treatment with Cd and 3-MA could notably elevate Caspase-3, Cyt C, Bax, and Bak-1 mRNA levels, Caspase-3 and cleaved Caspase-3 protein levels, and cell apoptotic rate as well as cell damage, decreased mitochondrial membrane potential (MMP), Bcl-2 mRNA level and the ratio of Bcl-2 to Bax compared to treatment with Cd alone. Overall, these results indicate Cd exposure can induce autophagy in duck renal tubular epithelial cells, and inhibition of autophagy might aggravate Cd-induced apoptosis through mitochondria-mediated pathway.Degradation of betrixaban, an oral anticoagulant recently approved by the U.S. Food and Drug Administration (FDA), and its N-nitrosodimethylamine (NDMA) formation potential were studied mechanistically in the presence of monochloramine (NH2Cl), free chlorine, and ozone. Upon monochloramination, the formation of NDMA exceeded 1% at basic pH and was significant at circumneutral pH as well. The kinetic studies revealed that the reaction between betrixaban and monochloramine followed pseudo-first-order reaction kinetics. Increasing monochloramine concentration, its reaction time, and pH all significantly enhanced the NDMA formation yield, which also increased three-fold in the presence of bromide during monochloraminantion. The presence of nitrite inhibited the formation of NDMA under the same conditions. This study revealed a new potent and significant precursor of NDMA, indicating that monochloramination of waters containing betrixaban can lead to the formation of NDMA and other disinfection by-products such as dichloroacetonitrile (DCAN) and dimethylformamide (DMF). Moreover, chlorination of betrixaban by hypochlorite also yielded NDMA, albeit at two orders of magnitude lower yield than chloramination, while no NDMA formation was observed from ozonation of betrixaban.Perfluorochemicals (PFCs) are a set of chemicals containing C-F bonds, which are concerned due to their bioaccumulation property, persistent and toxicological properties. Photocatalytic approaches have been widely studied for the effective removal of PFCs due to the mild operation conditions. This review aims to provide a comprehensive and up-to-date summary on the homogenous and heterogeneous photocatalytic processes for PFCs removal. Specifically, the homogenous photocatalytic methods for remediating PFCs are firstly discussed, including generation of hydrated electrons (eaq‒) and its performance and mechanisms for photo-reductive destruction of PFCs, the active species responsible for photo-oxidative degradation of PFCs and the corresponding mechanisms, and metal-ion-mediated (Fe(III) mainly used) processes for the remediation of PFCs. The influences of molecular structures of PFCs and water matrix, such as dissolved oxygen, humic acid, nitrate, chloride on the homogenous photocatalytic degradation of PFCs are also discussed. For heterogeneous photocatalytic processes, various semiconductor photocatalysts used for the decomposition of perfluorooctanoic acid (PFOA) are then discussed in terms of their specific properties benefiting photocatalytic performances. The preparation methods for optimizing the performance of photocatalysts are also overviewed. Moreover, the photo-oxidative and photo-reductive pathways are summarized for remediating PFOA in the presences of different semiconductor photocatalysts, including active species responsible for the degradation. We finally put forward several key perspectives for the photocatalytic removal of PFCs to promote its practical application in PFCs-containing wastewater treatment, including the treatment of PFCs degradation products such as fluoride ion, and the development of noble-metal free photocatalysts that could efficiently remove PFCs under solar light irradiation.Ozone has a strong oxidation power that allows effective inactivation of waterborne viruses. BRD7389 Few studies have accurately measured the kinetic relationship between virus inactivation and ozone exposure, because the high reactivity of ozone makes it difficult to measure them simultaneously. A continuous quench flow system (CQFS) is a possible solution for analyzing such a fast reaction; however, previous studies reported that CQFS provided different results of inactivation rate constants from the batch system. The objectives of this study were (1) to develop a CQFS to evaluate the kinetics of microbial inactivation accurately, (2) to evaluate the inactivation rate constants of waterborne virus by ozone, and (3) to compare the results with previous studies. The results indicated that the simple plug flow assumption in the reaction tube of CQFS led to underestimation of the rate constants. The accurate measurement of rate constants was achieved by the pseudo-first-order reaction model that takes the residence time distribution (RTD; i.e., the laminar flow assumption) into account. The results of inactivation experiments suggested that the resistance of viruses were getting higher in the following order Qβ less then MS2, fr, GA less then CVB5 Faulkner, φX-174, PV1 Sabin, CVB3 Nancy. The environmental isolates of CVB3 and CVB5 had a 2-fold higher resistance compared with their lab strains. Predicted CT values for 4-log inactivation ranged from 0.018 mg sec L-1 (Qβ) to 0.31 mg sec L-1 (CVB3 Environmental strain). The required CT values for 4-log PV1 inactivation was 0.15 mg sec L-1, which was 166-fold smaller than those reported in the United States Environmental Protection Agency guidance manuals. The overestimation in previous studies was due to the sparse assumption of RTD in the reactor. Consequently, the required ozone CT values for virus inactivation should be reconsidered to minimize the health risks and environmental costs in water treatment.The membrane-aerated biofilm reactor (MABR) is an emerging wastewater treatment technology that uses O2-supplying membranes as a biofilm support. Because O2 is supplied from the biofilm base instead of the bulk liquid, MABR biofilms have distinct microbial community structures and behavior. Past research showed that protozoan predation in MABR biofilms can greatly increase biofilm porosity, producing a void layer at the base of the biofilm. We hypothesized that this void layer could weaken the biofilm and promote sloughing, and investigated this with heterotrophic MABR biofilms. A rheometer was used to measure biofilm mechanical strength, and MABR flow cells were used to explore detachment. MABRs supplied with cycloheximide, a protozoan inhibitor, were used as controls. Predation increased the internal void ratio from 6 ± 7% to 50 ± 16%. The storage modulus was 1,780 ± 1,180 Pa with predation condition, compared to 9,800 ± 4,290 Pa for the control. Similarly, the loss modulus was 1,580 ± 729 Pa with predation and 363 ± 189 Pa for the control.