Silver-doped ZnO nanorods (Ag/ZnO) arrays have in-situ grown onto indium tin oxide (ITO) via the one-pot hydrothermal route towards a highly selective and recyclable electroanalysis of phosphothioate pesticides (PTs) with phoxim (Phox) as a model. It was discovered that the Ag/ZnO arrays-modified electrode could obtain a steady and sharp electrochemical output of solid-state Ag/AgCl at a low potential (i.e., 0.12 V). More importantly, the achieved Ag/AgCl signals could decrease selectively induced by sulfide (S)-containing Phox by the specific Cl-S displacement reaction, which would trigger AgCl into non-electroactive Ag-Phox complex. Mezigdomide The Ag/ZnO arrays-modified sensors present a linear range from 0.050 to 700.0 μM for the detection of Phox, with a limit of detection down to 0.010 μM. The practical applicability of the developed electroanalysis strategy was successfully employed to detect Phox in the tap water and cabbage samples. Moreover, the photocatalytic performances of the Ag/ZnO arrays were subsequently verified for the degradation of Phox, displaying the higher photocatalytic efficiency than pure ZnO nanorods. Besides, the as-developed sensor can allow for the recyclable detection of Phox by the Ag/ZnO-photocatalyzed removal of Phox after each of the detection cycles. Therefore, the sensors platform based on Ag/ZnO arrays can be expected to have potential for the electrochemical monitoring and photocatalytic degradation of toxic pesticides in the food and environmental fields.Gold plasmon nanoparticle (AuNP) was applied to the detection and the quantification of pollutant Cr(III) in water. It was synthesized by the chemical reduction of tetrachloroauric(III) acid with sodium citrate as a reducing and capping agent and was modified with 3-mercaptopropanoic acid (3-mpa) to improve the sensing recognition for the metal ion in the colorimetric detection. The 3-mpa-deposited AuNP selectively bound Cr(III) among the other 14 metal cations, resulting in the redshift of the gold plasmon band from 521 nm to 670 nm. The colorimetric quantification examination of the Cr(III) using the plasmon intensity approved the high sensitivity with the low limit of detection (0.34 ppb). Meanwhile, for the electrochemical detection, AuNP was electrochemically deposited on indium tin oxide glass substrate, modified with 3-mpa, attached Cr(III), and subsequently capped with 3-mpa-deposited AuNP. The cathodic current peak at -0.84 V versus the metal ion concentration revealed the linearity at a wide concertation range of 200-5000 ppb. As a result, the proposed colorimetric and electrochemical sensing techniques, which are the simple and facile detectors, can be complementarily employed with a high selectivity, sensitivity and wide analyte concentration range for the quantification of Cr(III) in aqueous solutions.Cysteine (Cys) is an indispensable small organic molecule containing sulfhydryl groups, which has essential regulatory effects on the physiological process of human body. In this work, a red emission fluorescent probe TCFQ-Cys was designed and exploited based on 2-(3-cyano-4,5,5-trimethylfuran-2(5H)-ylidene) malononitrile-derivatives. The probe could effectively monitor Cys through the typical acrylate cleavage. The detecting system showed a red emission at 633 nm and the fluorescence was stable within the pH range of 6-9. The detection could be completed in 30 min. TCFQ-Cys presented high sensitivity with a detection limit of 0.133 μM and high selectivity towards Cys from other biological mercaptans. The most important feature was that the system had a wide linear range of 0-300 μM, which covered the physiological requirements of Cys detection. Subsequently, we conducted the biological imaging of Cys in MCF-7 cells and Caenorhabditis elegans (C. elegans). Therefore, TCFQ-Cys had a practical application prospect for further investigating the physiological function of Cys.In this series of two papers, 192 doping agents belonging to the classes of stimulants, narcotics, cannabinoids, diuretics, β2-agonists, β-blockers, anabolic agents, and hormone and metabolic modulators were investigated, with the aim to assess the benefits and limitations of ion mobility spectrometry (IMS) in combination with ultra-high performance liquid chromatography (UHPLC) and high resolution mass spectrometry (HRMS) in anti-doping analysis. In this first part, a generic UHPLC-IM-HRMS method was successfully developed to analyze these 192 doping agents in standard solutions and urine samples, and an exhaustive database including retention times, TWCCSN2 values, and m/z ratios was constructed. Urine samples were analyzed using either a simple “dilute and shoot” procedure or a supported liquid-liquid extraction (SLE) procedure, depending on the physicochemical properties of the compounds and sensitivity criteria established by the World Anti-Doping Agency (WADA) as the minimum required performance levels (MRPL). Then, the precision of the generic UHPLC-IM-HRMS method was assessed as intraday, interday as well as interweek variation of UHPLC retention times and TWCCSN2 values, for which RSD the values were always lower than 2% in urine samples. The possibility to filter MS data using IMS dimension was also investigated, and in average, the application of IMS filtration provided low energy MS spectra with 86% less interfering peaks in both standard and urine samples. Therefore, the filtered MS spectra allowed for an easier interpretation and a lower risk of false positive result interpretations. Finally, IMS also offers additional selectivity to the UHPLC-HRMS enabling to separate isobaric and isomeric substances. Among the selected set of 192 doping agents, there were 30 pairs of isobaric or isomeric compounds, and only two pairs could not be resolved under the developed conditions. This illustrates the potential of adding ion mobility to UHPLC-HRMS in anti-doping analyses.Single atom nanomaterials possess catalytic activity like natural enzymes are termed as SAzymes which have gained great attention during last two years because of the maximal utilization of atoms and the benefit of understanding structure-property relationship. However, most of SAzymes are fabricated based on hydrophobic carbon, which disperse poorly in water and exhibit inferior affinity towards substrates, which may limit their biomedical applications. Here, we report a peroxidase-like SAzyme through the post-modification route based on hydrophilic defective metal-organic frameworks. Hydrochloric acid (HCl) is employed as ligand modulator to fabricate defective NH2-UiO-66 nanoparticles (HCl-NH2-UiO-66 NPs). Compared with the NPs fabricated through acetic acid modulation method (Ac-NH2-UiO-66 NPs), HCl-NH2-UiO-66 NPs have more missing linkers. Hence, more Fe(Ⅲ) ions can be successfully doped onto Zr6 clusters in HCl-NH2-UiO-66 NPs in a single atom state via formation of Fe-O-Zr bridge. The HCl-NH2-UiO-66 NPs doped with Fe(Ⅲ) ions (Fe-HCl-NH2-UiO-66 NPs) possess higher peroxidase-like activity than Fe-Ac-NH2-UiO-66 NPs due to the higher loading amount of Fe.