This new bisulphite-free ultra-low input methylation profiling technology has great potential in non-invasive and cost-effective cancer detection and classification.Glucagon-Like Peptide-1 (GLP-1) is an important peptide hormone secreted by L-cells in the gastrointestinal tract in response to nutrients. It is produced by the differential cleavage of the proglucagon peptide. GLP-1 elicits a wide variety of physiological responses in many tissues that contribute to metabolic homeostasis. For these reasons, therapies designed to either increase endogenous GLP-1 levels or introduce exogenous peptide mimetics are now widely used in the management of diabetes. SP-13786 PREP inhibitor In addition to GLP-1 production from L-cells, recent reports suggest that pancreatic islet alpha cells may also synthesize and secrete GLP-1. Intra-islet GLP-1 may therefore play an unappreciated role in islet health and glucose regulation, suggesting a potential functional paracrine role for islet-derived GLP-1. In this review, we assess the current literature from an islet-centric point-of-view to better understand the production, degradation, and actions of GLP-1 within the endocrine pancreas in rodents and humans. The relevance of intra-islet GLP-1 in human physiology is discussed regarding the potential role of intra-islet GLP-1 in islet health and dysfunction.Few studies exist that examine within-group differences of the transgender population. This paper aims to assess differences in sociodemographic characteristics and health-related quality of life (HRQoL) among transgender men (TM), transgender women (TW), and transgender gender nonconforming (TGNC) individuals in the United States. Data were pooled from the 2014-2017 Behavioral Risk Factor Surveillance System. Chi-square tests and weighted multivariable logistic regression models investigated differences in HRQoL by gender identity and separately identified predictors of HRQoL in these groups. Overall, TGNC individuals self-reported worse general health than TW or TM. TW and TGNC individuals who also identified as lesbian, gay, or bisexual (LGB) had higher odds of reporting frequent mental distress compared to their heterosexual counterparts. Race/ethnicity, education, and employment also emerged as predictors of HRQoL. Findings support the need to disaggregate these subpopulations of transgender individuals to address the unique needs of each subgroup.Thousands of human deaths occur annually due to Japanese encephalitis (JE), caused by Japanese encephalitis virus. During the virus infection of the central nervous system, reactive gliosis, uncontrolled inflammatory response, and neuronal cell death are considered as the characteristic features of JE. To date, no specific treatment has been approved to overcome JE, indicating a need for the development of novel therapies. In this article, we focused on basic biological mechanisms in glial (microglia and astrocytes) and neuronal cells that contribute to the onset of neuroinflammation and neuronal cell damage during Japanese encephalitis virus infection. We also provided comprehensive knowledge about anti-JE therapies tested in clinical or pre-clinical settings, and discussed recent therapeutic strategies that could be employed for JE treatment. The improved understanding of JE pathogenesis might lay a foundation for the development of novel therapies to halt JE.Abbreviations AKT a serine/threonine-specific pr PTEN phosphatase and tensin homolog; Rab7 Ras-related GTPase 7; Raf proto-oncogene tyrosine-protein kinase Raf; Ras a GTPase; RIDD regulated IRE-1-dependent decay; RIG-I retinoic acid-inducible gene I; RIPK1/3 receptor-interacting protein kinase 1/3; RNF11/125 RING finger protein 11/125; ROS reactive oxygen species; SHIP1 SH2-containing inositol 5′ phosphatase 1; SOCS5 suppressor of cytokine signaling 5; Src proto-oncogene tyrosine-protein kinase Src; ssRNA = single-stranded RNA; STAT signal transducer and activator of transcription; TLR toll-like receptor; TNFAIP3 tumor necrosis factor alpha-induced protein 3; TNFAR tumor necrosis factor alpha receptor; TNF-α tumor necrosis factor-alpha; TRAF6 tumor necrosis factor receptor-associated factor 6; TRIF TIR-domain-containing adapter-inducing interferon-β; TRIM25 tripartite motif-containing 25; VCAM vascular cell adhesion molecule; ZO-1 zonula occludens-1.This study aimed to develop and evaluate a novel observational technique for postural Loading on the Entire Body Assessment (LEBA). The technique was developed based on discomfort and epidemiological data from previous research, from which posture classification and scoring systems of representative observational methods were adopted and modified. The LEBA score reflected the effects of posture, external load, motion repetition, static loading, and coupling. The LEBA score for a given posture was obtained by summing the scores for these factors (except coupling) and multiplying the sum by the coupling multiplier. LEBA scores were classified into four action categories, depending on the urgency of corrective actions. Correlation analyses between LEBA scores and postural load criteria yielded confirmative results, with correlation coefficients of >0.60. Application to epidemiological cases of work-related musculoskeletal disorders indicated that LEBA action categories aided in determining whether musculoskeletal disorders were work-related. Acceptable reliability and usability were also observed.Practitioner Summary This study developed and evaluated a novel observational technique for postural loading on the entire body assessment (LEBA), based on perceived discomfort and epidemiological data from previous studies. LEBA scores aided in determining risk levels and urgent indications for more detailed assessments and/or interventions and the work-relatedness of musculoskeletal disorders.Dual process models posit that combinations of impulsive and reflective processes drive behaviour, and that the capacity to engage in effortful cognitive processing moderates the relation between measures of impulsive or reflective processes and actual behaviour. When cognitive resources are low, impulsive processes are more likely to drive behaviour, while when cognitive resources are high, reflective processes will drive behaviour. In our current study, we directly addressed this hypothesis by comparing the capacity of implicit and explicit measures to predict fear and anxiety, either with or without additional cognitive load. In Experiment 1 (N = 83), only explicit measures of spider fear were predictive of spider avoidance, and manipulating cognitive load did not affect these relations. Experiment 2 (N = 70) confirmed these findings, as the capacity of explicit and implicit measures to predict self-reported and physiological responses to a social stressor was not moderated by cognitive load. In two experiments, we thus found no empirical support for the central dual process model assumption that cognitive control moderates the predictive value of implicit and explicit measures.